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This document describes a set of physiological parameters allowing the measurement of sublethal effects in higher plants exposed to soil pollutants and other stressors (e.g. drought, nutrient availability, heat stress). It is applicable to soils of unknown quality (e.g. from contaminated sites, amended soils or soils after remediation), either in situ or following laboratory exposure assays. This document specifies the methods for analysing variations in physiological responses and oxidative damage that can be indicative of stress symptoms in the leaves of higher plants, either monocotyledonous or dicotyledonous species.