Kemesta

This document specifies a method for the detection and identification of microalgae, macroalgae (seaweed), cyanobacteria and Labyrinthulomycetes by using morphological methods and/or molecular methods.The morphological methods in this document are applicable to harvested wet biomass and to harvested dried unground biomass from microalgae, macroalgae, cyanobacteria and Labyrinthulomycetes that have been grown and/or harvested for further processing and/or use.The molecular methods in this document are applicable to harvested wet biomass and to harvested dried and/or ground biomass from microalgae, macroalgae, cyanobacteria and Labyrinthulomycetes that have been grown and/or harvested for further processing and/or use.This document describes a toolbox, consisting of several identification methods that can be chosen according to the applicability and purpose of the identification:— morphological methods based on observation and referring to scientific literature on taxonomy:— macroscopic observation;— light microscopic observation;— molecular methods of sequencing and blasting of sequences:— 16S-rDNA sequencing;— 18S-rDNA sequencing;— rbcL DNA sequencing;— ITS sequencing;— COX 1 gene sequencing;— tufA gene sequencing.This document does not deal with genetic purity of the biomass or quantification of the identified taxa.