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This document was developed to provide the methodology for the enumeration and determination of Azotobacter sp. in plant biostimulant products in accordance with the Regulation (EU) 2019/1009 of the European Parliament and of the Council [1].

This document defines general rules for the aerobic preparation of the initial suspension and of dilutions for microbiological examinations of microbial plant biostimulants.This horizontal method might not be appropriate in very detail for certain products. In this case, different methods which are specific to these products can be used if necessary, for justified technical reasons.

This document specifies the general principles for justifying the product claims for plant biostimulants.General principles consist of and define all general parameters, requirements and quality criteria, and are intended to be applied in order to assess the efficacy of trials used for claim(s) validation as a result of the use of a plant biostimulant.This document is aimed primarily at manufacturers, laboratories, researchers, technical centres, companies that will put the products on market, notifying authorities, notified bodies, and market surveillance authorities.

This document provides guidance for justifying quality traits claims of plant biostimulants used in agriculture.This document is aimed primarily at manufacturers, laboratories, companies which will put the products on market, notifying authorities, notified bodies, and market surveillance authorities.

This document specifies a horizontal method for the enumeration of yeasts and moulds present in plant biostimulant intended for use in agriculture, by means of the colony count technique after aerobic incubation at 25 °C +- 2,5 °C.This document allows the enumeration of yeasts and moulds, in technical and formulated plant biostimulant, both in liquid and solid state. The method is applicable to microbial plant biostimulant except those composed of fungi or yeast to verify that the concentration of yeast and moulds does not exceed the respective limits described in the EU Fertilizers Regulation [1].If necessary, yeast and mould enumerated can be identified using suitable identification tests.

This document specifies methods for the reduction and preparation of samples of non-microbial plant biostimulants including those intended for determination of microbial pathogens and sets out the requirements for sample preparation reports. It specifies methods for the preparation of test samples and test portions from laboratory samples of plant biostimulants for subsequent chemical, biological or physical analysis.It is also applicable to the sample preparation of blends of fertilizing products where plant biostimulants are main part of the blend. Otherwise, deliverables of sample preparation relevant for the main part of the blend apply.This document does not include methods for the reduction and preparation of samples of microbial plant biostimulants, which will be covered by a different Technical Specification.NOTE This document is applicable to the category of EU fertilizing product (plant biostimulants) in the meaning of the Regulation (EU) 2019/1009.

This document specifies sampling plans and methods of representative sampling of plant biostimulants to obtain samples for physical, chemical and biological analysis.It is applicable to the sampling of lots of plant biostimulants supplied or ready for supply to third parties, as such, or in smaller lots.It is also applicable to the sampling of blends of fertilizing products where plant biostimulants are main part of the blend. Otherwise, deliverables of sampling relevant for the main part of the blend apply.This document is intended to be used by manufacturers, buyers and competent authorities to obtain samples prior to transport and supply it to a laboratory for testing.NOTE This document is applicable to the category of EU fertilizing product (plant biostimulants) in the meaning of the Regulation (EU) 2019/1009.

This document specifies terminology for soil improvers and growing media.Annex A contains an overview of all terms defined in this document in alphabetical order.

This document provides an overview of relevant methods for the determination of specific parameters in growing media, including:- the electrical conductivity;- the pH;- dry matter;- the nitrogen, P2O5 and K2O content extractable by CaCl2/DTPA;- the total copper and zinc content;- the quantity.This document is applicable to EU fertilizing products classified as PFC 4 and PFC 7 as long as the main function of the EU fertilizing product is classified as PFC 4 of Regulation (EU) 2019/1009 [2].This document is not applicable to preformed materials such as mineral wool slabs and foam slabs.

This document provides an overview of relevant methods for the properties of compost and solid digestate when used in fertilizing products, including:- macroscopic impurities;- oxygen uptake rate;- self-heating factor.This document is applicable to the following component material categories: CMC 3, CMC 4 and CMC 5, as specified in the Regulation (EC) No 2019/1009 [2].

This document provides an overview of relevant methods for the determination of specific parameters in solid soil improvers, including:- dry matter content;- nitrogen content;- P2O5 and K2O content;- chloride, copper and zinc content;- quantity.This document is applicable to solid EU fertilizing products classified as PFC 3 and PFC 7 as long as the main function of the EU fertilizing product is classified as PFC 3 of Regulation (EU) 2019/1009 [2].

This document specifies references to methods for the determination of the following parameters:- pH;- electrical conductivity.This document is applicable to solid EU fertilizing products classified as PFC 3(A) and PFC 7 as long as the main function of the EU fertilizing product is classified as PFC 3(A) of Regulation (EU) 2019/1009 [2].

This document specifies a method for the extraction and determination of phosphonates (P-PO3) in plant biostimulants using ion chromatography and conductivity detection (IC-CD).This document is also applicable to the blends of fertilizing products where plant biostimulants are the main part of the blend. Otherwise, the Technical Specification for the main part of the blend apply.

This document specifies the procedure for the determination and calculation of the dry matter fraction of plant biostimulants for which the results of performed analysis are to be calculated to the dry matter basis.This document is also applicable to the blends of fertilizing products where plant biostimulants are the main part of the blend. Otherwise, the Technical Specification for the main part of the blend apply.

This document specifies a method for extraction, separation, and determination of inorganic arsenic (iAs) in plant biostimulants using anion-exchange HPLC or IC coupled to ICP-MS.This document is also applicable to the blends of fertilizing products where plant biostimulants are the main part of the blend. Otherwise, the Technical Specification for the main part of the blend apply.

This document specifies a method for determination of the content of mercury (Hg) in plant biostimulants using (cold) vapour generation apparatus coupled to an atomic absorption spectrophotometer and a method using a direct amalgamation technique. It is applicable to aqua regia digests prepared according to CEN/TS 17701-1.NOTE It is also possible to use other suitable methods for the determination of mercury described in Annex A if users prove that the method gives the same results as the methods described in this document.This document is also applicable to the blends of fertilizing products where plant biostimulants are the main part of the blend. Otherwise, the Technical Specification for the main part of the blend applies.

This document was developed to provide a method for verifying that hexavalent chromium (CrVI) is not present in plant biostimulants in a concentration that exceeds the respective limits outlined in the EU Regulation on Fertilising Products [1].This document is applicable to all types of plant biostimulants (solid and liquid ) used in agriculture.The method described is suitable to quantify the chromium(VI) content in plant biostimulants down to 2 mg/kg.The results obtained from this method are strictly dependent on the extraction conditions. Results obtained by using other extraction procedures (extraction solution, pH, extraction time, etc.) are not comparable with the results produced by the procedure described in this document.

This document specifies a method for the determination of arsenic (As), cadmium (Cd), copper (Cu), chromium (Cr), lead (Pb), nickel (Ni) and zinc (Zn) in plant biostimulant digests using inductively coupled plasma-atomic emission spectrometry (ICP-AES).This document is also applicable to the blends of fertilizing products where plant biostimulants are the main part of the blend. Otherwise, the Technical Specification for the main part of the blend applies.This method is applicable to aqua regia digests prepared according to CEN/TS 17701-1. The method can be used for the determination of other elements, provided the user has verified the applicability.

This document provides the methodology for the enumeration and determination of Rhizobium sp., Mesorhizobium sp., Ensifer sp., or Bradyrhizobium sp.) in plant biostimulant products in accordance with the Regulation (EU) 2019/1009 of the European Parliament and of the Council [1].

This document was provided to define general rules for determine microorganism concentration present in plant biostimulant products. The method is applicable to microbial plant biostimulants for verifying that the concentration of microorganisms does not exceed the respective limits outlined in the EU Regulation on Fertilising Products [1].This horizontal method might not be appropriate in very detail for certain products. In this case, it is necessary to refer to the methodology of specific determination and quantification of the microorganisms.